Wednesday, November 18, 2020

Bladder Cancer A Review

November 17, 2020 Andrew T. Lenis, MD, MS1Patrick M. Lec, MD1Karim Chamie; et alMD, MSHS1

Author Affiliations Article Information JAMA. 2020;324(19):1980-1991. doi:10.1001/jama.2020.17598

Abstract

Importance  Bladder cancer is a common malignancy in women and is the fourth most common malignancy in men. Bladder cancer ranges from unaggressive and usually noninvasive tumors that recur and commit patients to long-term invasive surveillance, to aggressive and invasive tumors with high disease-specific mortality.

Observations  Advanced age, male sex, and cigarette smoking contribute to the development of bladder cancer. Bladder tumors can present with gross or microscopic hematuria, which is evaluated with cystoscopy and upper tract imaging depending on the degree of hematuria and risk of malignancy. Non–muscle-invasive tumors are treated with endoscopic resection and adjuvant intravesical therapy, depending on the risk classification. Enhanced cystoscopy includes technology used to improve the detection of tumors and can reduce the risk of recurrence. Patients with high-risk non–muscle invasive tumors that do not respond to adjuvant therapy with the standard-of-care immunotherapy, bacille Calmette-Guérin (BCG), constitute a challenging patient population to manage and many alternative therapies are being studied. For patients with muscle-invasive disease, more aggressive therapy with radical cystectomy and urinary diversion or trimodal therapy with maximal endoscopic resection, radiosensitizing chemotherapy, and radiation is warranted to curb the risk of metastasis and disease-specific mortality. Treatment of patients with advanced disease is undergoing rapid changes as immunotherapy with checkpoint inhibitors, targeted therapies, and antibody-drug conjugates have become options for certain patients with various stages of disease.

Conclusions and Relevance  Improved understanding of the molecular biology and genetics of bladder cancer has evolved the way localized and advanced disease is diagnosed and treated. While intravesical BCG has remained the mainstay of therapy for intermediate and high-risk non–muscle-invasive bladder cancer, the therapeutic options for muscle-invasive and advanced disease has expanded to include immunotherapy with checkpoint inhibition, targeted therapies, and antibody-drug conjugates.

Introduction

Bladder cancer accounts for an estimated 500 000 new cases and 200 000 deaths worldwide, and in the US alone there are more than 80 000 new cases and 17 000 deaths each year.1,2 It represents a spectra of diseases, from recurrent noninvasive tumors managed chronically, to aggressive or advanced-stage disease that requires multimodal and invasive treatment. Advances in understanding of the underlying biology of bladder cancer has fundamentally changed how this disease is diagnosed and treated.

The objective of this article is to provide an evidence-based review of the epidemiology, pathophysiology and molecular biology, diagnosis, and management of bladder cancer. Several guidelines have been published by the American Urological Association (AUA), the European Association of Urology (EAU), and the National Comprehensive Cancer Network (NCCN), which will be referenced herein and provide background information and management recommendations.

Methods

A literature review was conducted to address the epidemiology, risk factors, pathophysiology, molecular biology, presentation, diagnosis, and management of the various forms of bladder cancer. A formal systematic review was not performed, but relevant literature was identified by searching PubMed for English-language articles from inception through April 2020. Preference was given to articles that reported findings from meta-analyses and large randomized clinical trials (RCTs).

Data presented regarding management was compiled from the AUA, EAU, and NCCN guidelines.3-8 The strength of the recommendation and quality of supporting evidence varies with each organization and was reported herein as follows: guideline, strength/level of evidence. The AUA guideline recommendations are “strong,” “moderate,” or “conditional,” based on A (high), B (moderate), or C (low) levels of evidence. The EAU recommendations are “strong” or “weak,” and the level of evidence was graded from 1 (high-quality systematic reviews and individual RCTs) to 5 (expert consensus), as per the Oxford Center for Evidence-Based Medicine.9 The NCCN recommendations are “preferred intervention,” “other recommended intervention,” or “useful in certain circumstances,” based on levels of evidence 1 (high-level evidence and uniform consensus) to 3 (major NCCN disagreement).

Epidemiology and Risk Factors

Scope of the Disease

The lifetime risk of bladder cancer is approximately 1.1% in men and 0.27% in women.1 Higher incidence is reported in Western societies, largely due to carcinogen exposure.10 The prevalence of bladder cancer is high, with more than 1.6 million living with the disease worldwide.1

Risk Factors

Advanced age is the greatest risk factor for bladder cancer, with an average age of diagnosis between 70 and 84 years.11 This is explained by exposure to carcinogens such as tobacco smoke and, less commonly, benzene chemicals and aromatic amines, combined with an age-related reduction in the ability to repair DNA.12 While non-Hispanic White persons have the highest age-adjusted incidence rates of bladder cancer (23.09 [95%CI, 22.97-23.21] per 100 000 person-years), African Americans have worse disease-specific outcomes and greater rates of unfavorable pathology.13,14 Men are diagnosed with bladder cancer with 3 to 4 times the frequency of women, traditionally attributed to exposures and lifestyle, but stasis of urine-containing carcinogens in men with prostatic enlargement and urinary retention may also increase risk.12,15 Hematuria in women is often attributed to infection, resulting in delays in the diagnosis of bladder cancer and, consequently, worse cancer-specific and overall survival in women.15 Recent studies have demonstrated the effects of hormone receptors and genomic differences in some bladder cancers in women compared with men, which may also partially account for survival differences.16

Cigarette smoking is an important modifiable exposure, with a population-attributable risk of approximately 50%.17 Occupational exposures include benzene dyes and factory chemicals. Chronic inflammatory conditions such as bacterial and Schistosoma hematobium (particularly common in Northern Africa) infections, chronic indwelling Foley catheters, and prior bladder augmentation result in increased cellular proliferation predisposing to urothelial malignancy.18,19 Pelvic radiation (eg, for prostate, rectal, or cervical cancer) and cyclophosphamide, an alkylating cytotoxic chemotherapy agent, both increase the risk of bladder cancer.20-25

Epidemiologic studies have also demonstrated a component of heredity in the development of urothelial cancer. In a series of more than 200 000 same-sex twin individuals the estimated percent familial risk was 9.9% for monozygotic twins and 5.5% for dizygotic twins.26 In a study of nearly 600 patients with bladder cancer, variants in germline DNA, which can result in inherited risk of bladder cancer, were identified in 14% of patients; 83% of these variants were in DNA-damage repair genes.27 Lynch syndrome is an example of an autosomal dominant genetic syndrome caused by a highly penetrable alteration in DNA mismatch repair genes.28 Among other tumors that may develop in patients with Lynch syndrome, the lifetime risk of urothelial tumors of the upper urinary tract is estimated to be 0.4% to 20%.29 Whether the risk of bladder cancer is increased, however, remains controversial.

Pathophysiology and Molecular Biology

Bladder cancer is a carcinoma of the urothelial, or “umbrella,” cells that line the lumen of the urinary bladder. Technically, urothelial carcinoma includes tumors of the bladder, upper urinary tract (renal pelvis and ureters), and proximal urethra. Bladder cancer accounts for approximately 90% to 95% of urothelial carcinoma and is the focus of this review. Histologically, bladder cancer comprises 75% pure urothelial carcinoma and 25% “variant” histology, adding complexity to the management of this disease.30

Bladder cancer can be categorized in several ways. It is classified into high-grade vs low-grade disease based on standardized histomorphologic features as described by the World Health Organization. Tumor stage is assigned as a measure of depth of bladder wall invasion (Table 1). Tumors isolated to the urothelium (stage Ta) and the lamina propria (stage T1) are considered non–muscle-invasive bladder cancer (NMIBC) and are treated differently from tumors that invade the muscle (stage T2) or beyond (stages T3 and T4), called muscle-invasive bladder cancer (MIBC). Carcinoma in situ (CIS) is a distinct phenotype defined as a high-grade flat noninvasive lesion with particularly high rates of recurrence and progression.

Underlying these phenotypes are genetic alterations at the DNA and subsequent RNA expression level, forming distinct molecular subtypes that have prognostic, predictive, and therapeutic implications. Initial insights included the identification of high mutational burden of bladder cancer, similar to melanoma and lung cancer, providing a biologic basis for its response to immunotherapy.31 Independent efforts from multiple groups have identified mutations common in low-grade NMIBC (FGFR3, PIK3CA, STAG2, RTK/RAS/RAF pathway genes) and high-grade MIBC/advanced disease (ERBB2, p53, RB1, MDM2, CDKN2A, KDM6A, ARID1A). Tumors may be categorized into molecular subtypes (eg, luminal, basal/squamous) that inform clinical behavior such as response to neoadjuvant chemotherapy, sensitivity to immunotherapy, and risk of progression.32

Presentation and Diagnosis

The most common presentation of bladder cancer is visible, or gross, hematuria, but patients can also present with isolated microscopic hematuria (urinalysis showing ≥3 red blood cells per high-power field), irritative voiding symptoms, or a tumor incidentally discovered on imaging. The risk of bladder cancer is approximately 4% in patients with microscopic hematuria and 16.5% in those with gross hematuria.33 A guideline-recommended workup is presented in Table 2Evaluation of gross hematuria involves visualization of the bladder with cystoscopy and imaging of the upper urinary tract (ie, kidney, renal pelvis, and ureter) with cross-sectional urography. Updated microscopic hematuria evaluation guidelines from the AUA now recommend workup based on the risk of having bladder cancer, as compared with prior guidelines that indiscriminately recommended cross-sectional imaging.5 This change reflects the relatively low frequency of malignancy found on evaluation combined with the invasive nature of cystoscopy, radiation exposure from cross-sectional imaging, and the associated health care costs of obtaining these procedures.

Cystoscopy and Endoscopic Resection

Patients who may have bladder cancer undergo cystoscopy to evaluate the lower urinary tract. Cystoscopy is an office-based procedure performed with a flexible camera approximately 5 mm in diameter inserted via the urethra. Enhanced cystoscopy with narrow-band imaging or blue light cystoscopy offer improved sensitivity and specificity for identifying bladder tumors during diagnostic cystoscopy and endoscopic resection. Narrow-band imaging improves the detection rate (approximately 10% and 20% on a per-patient and per-lesion basis, respectively) and decreases the risk of recurrence at 3 and 12 months.34 Blue light cystoscopy detects up to 14% of papillary Ta/T1 lesions and 40% of CIS lesions missed on conventional cystoscopy.35

Endoscopic resection, or transurethral resection of bladder tumor (TURBT), of newly identified bladder tumors is diagnostic and potentially therapeutic. Its purpose is 2-fold—to resect all visible tumors and perform disease staging.3 In the case of an incomplete resection, lack of detrusor muscle in the pathologic specimen, or pathologic high-risk stage Ta or T1 disease, repeat TURBT is recommended within 4 to 6 weeks. Repeating the TURBT under these circumstances is critical, because there is a 51% rate of residual disease in patients with T1 disease and an 8% rate of upstaging from T1 to muscle-invasive disease with repeated procedures.36 Improving TURBT technique is a focus of quality improvement in bladder cancer care, as evidence links quality of resection to clinical outcomes.37 A retrospective cohort study of 1865 patients who underwent TURBT for bladder cancer found that an inadequate staging resection was associated with poorer cancer-specific survival (hazard ratio [HR], 1.48 [95% CI, 1.00-2.18]).37

Imaging

Cross-sectional urography (ie, computed tomography or magnetic resonance imaging urogram) is used to evaluate the upper urinary tract of patients with gross hematuria and high-risk microscopic hematuria, while renal ultrasound is used in patients with low- and intermediate-risk microscopic hematuria (Table 2). Although patients suspected of having bladder cancer are not routinely evaluated with imaging for the purpose of initial staging, a novel multiparametric system, VI-RADS (Vesical Imaging-Reporting and Data System), may be used to identify muscle invasive disease. In a meta-analysis of 6 studies with more than 1000 patients, the pooled sensitivity and specificity for detection of MIBC was 0.90 (95% CI, 0.86-0.94) and 0.86 (95% CI, 0.71-0.94).38

Urine Tests and Biomarkers

Urine cytology is used in the evaluation of gross hematuria and in posttreatment surveillance. The test involves a pathologist’s survey of sloughed primarily high-grade malignant urothelial cells, which lose their adhesive properties more readily than low-grade malignant cells. In a recent meta-analysis, the pooled sensitivity of urine cytology was 0.37 (95% CI, 0.35-0.39) and pooled specificity was 0.95 (95% CI, 0.94-0.95).39 While several urine biomarkers are approved by the US Food and Drug Administration (FDA) and are useful adjuncts in select patients, all lack the diagnostic accuracy required to replace cystoscopy.3 Detection of circulating tumor cells has been associated with clinical outcomes such as cancer-specific survival (HR, 5.18 [95% CI, 2.21-12.13]) and detection of cell-free tumor DNA with outcomes such as metastatic relapse (100% sensitivity, 98% specificity).40,41

Management

Non–Muscle-Invasive Bladder Cancer

NMIBC represents approximately 70% of organ-confined bladder cancer.42 It comprises a wide spectrum of disease, and the AUA has stratified patients into low-, intermediate-, and high-risk categories (Table 3 and Table 4). Although survival is favorable, patients with low- and intermediate-risk NMIBC experience 5-year recurrence-free survival rates of 43% and 33%, respectively, and up to 21% with high-risk disease will progress to MIBC (Box).43,44 Patients with low-risk disease who undergo a complete initial resection are managed with cystoscopic surveillance. Patients with high-grade stage Ta (AUA, moderate/C) or T1 (AUA, strong/B; EAU, strong/2) disease should undergo a repeat resection, given the risk of understaged or persistent disease in 17% to 67% of stage Ta tumors and 20% to 71% of stage T1 tumors.3,6 Barring upstaging to muscle-invasive disease on repeat resection, patients with intermediate-risk (AUA, moderate/B; EAU, strong/1a) and high-risk (AUA, strong/B; EAU, strong/1a) disease should receive a regimen of intravesical therapy.

Intravesical Therapy

Perioperative Chemotherapy

When low- or intermediate-risk disease is suspected, a single dose of intravesical chemotherapy may be administered within 24 hours of TURBT with the goal of killing free-floating tumor cells, thereby mitigating seeding of the urothelium (AUA, moderate/B; EAU, weak/1a).3 A meta-analysis of 7 randomized trials demonstrated a 39% reduction in the odds of tumor recurrence (absolute decrease from 48% to 37%) with intravesical chemotherapy at a median follow-up of 3.4 years.45 Mitomycin C has long been the agent of choice in this setting, but it is associated with irritative voiding symptoms and rare bladder necrosis and is expensive.46 More recently, gemcitabine has emerged as a viable alternative with similar efficacy and improved tolerability at a lower cost.47 Additional intravesical chemotherapeutic agents are available, including valrubicin and epirubicin; however, no randomized data compare efficacy of the above agents with one another.

Bacille Calmette-Guérin

Bacille Calmette-Guérin (BCG), a live attenuated form of Mycobacterium bovis, is the preferred treatment for high-risk NMIBC and an option for intermediate-risk NMIBC. The precise mechanisms by which this form of immunotherapy exerts its effects are complex. It has been posited that BCG adheres to the urothelium, is internalized, then induces antigen-presenting, cell-mediated induction of innate and adaptive immune responses.48 It is instilled into the bladder for 90 to 120 minutes, once weekly for 6 weeks, during an induction phase. If the patient tolerates and responds to treatment, as assessed by postinduction cystoscopy, a maintenance regimen should be continued for 1 year (AUA, moderate/C for intermediate risk) to 3 years (AUA, moderate/B for high risk).3 Contemporary rates of complete response are near 80% after induction and are durable at near 55% at 3 years. A meta-analysis of patients with CIS demonstrated that BCG improved the recurrence-free rate from 26% to 47% (odds ratio, 0.41 [95% CI, 0.30-0.56]) and the progression-free rate from 20% to 15% (OR, 0.74 [95% CI, 0.45-1.22]) compared with various intravesical chemotherapy agents.49 Rates of adverse effects are common (up to 60% in some series) and can manifest as chemical cystitis, irritative voiding symptoms, and malaise.50 In a small fraction of patients (1%), systemic absorption of BCG may lead to severe sepsis and require treatment with antibiotics, steroids, and cessation of BCG therapy.

BCG Shortage, Failure, and Alternative Therapies

Despite its preferred status, BCG has become difficult to obtain in the US due to supply chain interruptions.51 Regulatory hurdles, financial disincentives for pharmaceutical companies, and manufacturing challenges all contribute to decreased production amidst an increasing demand for BCG.52 In patients for whom urologists are unable to obtain BCG or for patients with BCG therapy failure, few alternatives exist. For any patient with BCG failure, radical cystectomy should be considered. One FDA-approved option for patients with CIS after BCG therapy is valrubicin; however, only 20% of patients are tumor-free at 12 months and less than 10% remain so beyond 12 months.53

Sequential gemcitabine-docetaxel is also used by some clinicians. In a retrospective cohort study of 276 patients with recurrent NMIBC, prior BCG therapy, and median follow-up of 22.9 months, this combination provided durable recurrence-free survival rates of 60% at 1 year and 46% at 2 years.54 Recently, the FDA approved the systemic checkpoint inhibitor pembrolizumab, based on KEYNOTE-057, a phase 2 single-group clinical trial in patients with high-risk BCG-unresponsive NMIBC showing a complete response rate of 41%; only 46% of complete responders (about 19%) maintained this response at 12 months.55 Given the need for additional therapies for BCG-unresponsive disease, the FDA may consider approval for therapeutics based on single-group studies that demonstrate approximately 30% response at 12 months.56 Several promising intravesical therapies, such as nadoferagene firadenovec (a nonreplicating recombinant adenovirus carrying the IFNα2b gene), vincinium (an epithelial cell adhesion molecule gene [EPCAM]–targeted Pseudomonas exotoxin A), and ALT-803 (an IL-15 superagonist), have demonstrated some efficacy in patients with BCG-unresponsive disease.51

Muscle-Invasive Bladder Cancer

Muscle-invasive bladder cancer represents the remaining 30% of localized disease. Treatment for patients with MIBC consists of neoadjuvant therapy followed by radical cystectomy, pelvic lymph node dissection, and urinary diversion, or a bladder-sparing protocol, such as chemoradiation or partial cystectomy, in selected patients (Table 5).4 Strategies to improve adherence and enhance tolerability of treatment are critical to improve outcomes for patients with MIBC, as reports demonstrate that more than one-half of patients do not receive curative-intent treatment.57

Neoadjuvant Systemic Therapy

Neoadjuvant chemotherapy prior to radical cystectomy is recommended in both the AUA (strong/B) and EAU (strong/1a) guidelines.4,7 In the pivotal Southwest Oncology Group 8710 randomized trial, an absolute improvement in survival of 31 months was demonstrated with the addition of neoadjuvant chemotherapy prior to cystectomy.58 A meta-analysis of 15 prospective studies included more than 3000 patients who received neoadjuvant chemotherapy prior to local therapy with surgery or radiation and showed an overall survival benefit (HR, 0.87 [95% CI, 0.79-0.96]; P < .01).59 In a subgroup analysis of those who received cisplatin-based therapy, the absolute improvement in overall survival was 8% at 5 years. Current practice supports the use of accelerated or dose-dense methotrexate, vinblastine, doxorubicin, and cisplatin or gemcitabine and cisplatin, and an ongoing phase 3 study (VESPER) is actively comparing these regimens.60-62 Preliminary data from this trial demonstrate higher complete response rates in patients receiving dose-dense methotrexate, vinblastine, doxorubicin, and cisplatin (MVAC) vs gemcitabine + cisplatin (42% vs 36%, P = .02). Up to 50% of patients will not be cisplatin-eligible for 1 or more of the following reasons: Eastern Cooperative Oncology Group performance status 2 or greater, creatinine clearance less than 60mL/min, grade 2 or greater hearing loss, grade 2 or greater neuropathy, or New York Heart Association class III heart failure.63 Carboplatin, while tolerated with diminished kidney function, is an unacceptable alternative to first-line neoadjuvant chemotherapy because of poorer efficacy.64 Neoadjuvant systemic immunotherapy with pembrolizumab has shown promise in a prospective single-group phase 2 trial of 50 patients with MIBC, with 42% of patients achieving pathologic complete response and up to 54% downstaged to pT1 or lower disease.65 Updated results in 114 patients confirmed these findings but also demonstrated efficacy in patients with variant histology (particularly squamous cell and lymphoepithelioma-like carcinoma), who traditionally do not respond to neoadjuvant chemotherapy, likely given higher tumor mutational burden and PD-L1 expression in a subset of these patients.66 While pathologic complete responses can be achieved, not all patients benefit from neoadjuvant treatment; therefore, biomarkers such as molecular subtype and specific alterations in DNA damage repair genes may guide utilization.

Radical Cystectomy, Pelvic Lymph Node Dissection, and Urinary Diversion

Radical cystectomy involves removal of the bladder, prostate, and seminal vesicles in men and the uterus, fallopian tubes, ovaries, and anterior vagina in women. Lymph node dissection is essential during curative-intent radical cystectomy and is supported by the AUA (strong/B), the EAU (strong/3), and the NCCN. Lymph node dissection provides important prognostic information and guides adjuvant therapy, as up to 25% and 8% of patients with MIBC and high-risk NMIBC will harbor lymph node metastases at the time of radical cystectomy. Lymph node dissection may also provide a therapeutic benefit, as approximately 20% of patients with positive lymph nodes will have long-term survival with a meticulous dissection.67 A systematic review reported on 7 studies with more than 13 000 patients and showed an increase in the 5-year overall survival from 25% to 50%, to 64% to 68%, with the performance of lymph node dissection.68 However, the extent of lymph node dissection during cystectomy remains controversial, as the first prospective RCT found no benefit to extended lymph node dissection in patients undergoing cystectomy in 5-year recurrence-free survival (65% vs 59%; HR, 0.84; P = .36), cancer-specific survival (76% vs 65%; HR, 0.70; P = .10), or overall survival (59% vs 50%; HR, 0.78; P = .12).69 The conclusions drawn by that trial can be contested, however, because of the large number of lymph nodes removed in both the limited and extended lymph node dissection cohorts (obscuring comparisons between low and high lymph node removal), the exclusion of patients receiving neoadjuvant chemotherapy, as well as the inclusion of patients with high-grade NMIBC, who are less likely to have lymph node–positive disease. Another active trial (SWOG 1011 [NCT01224665]) addresses this potential weakness by excluding patients at lower risk of lymph node metastases, such as those with NMIBC. However, that trial allows for neoadjuvant chemotherapy and a less aggressive dissection in the extended lymphadenectomy group that may mitigate potential differences in outcomes between groups.

Urinary diversion at time of cystectomy may take several forms: an incontinent ileal conduit, an orthotopic neobladder, or a continent cutaneous diversion (Figure).70 Selection of the urinary diversion type should come from a well-informed discussion of the risks, benefits, and postoperative expectations between the patient, the family, and the surgeon and incorporates patient- and tumor-specific factors. In clinical practice, more than 80% of patients undergo an ileal conduit urinary diversion, given the frailty of the patient population, the relative familiarity of this operation to the majority of urologists, and a potentially reduced frequency of postoperative complications.71

Radical cystectomy series have demonstrated 90-day complication rates in nearly two-thirds of patients and mortality ranging from 1.5% to 2.0% at 30 days postoperatively.72,73 In large national databases and institutional series, readmission rates are approximately 25% within 30 days of discharge.74 Postoperative complications are most commonly gastrointestinal (29%), infectious (25%), wound-related (15%), and genitourinary (11%).72 There are many long-term sequelae of urinary diversion that should be considered in the primary management of the care of patients who have undergone cystectomy. Absorption of ammonium chloride and bicarbonate wasting in intestinal diversions result in metabolic acidosis, which may require bicarbonate supplementation. Use of long segments of ileum comes at the cost of physiologic vitamin B12 resorption, and consequently serum vitamin B12 levels should be monitored annually in these patients. Obstruction from ureterointestinal anastomotic strictures and urinary retention in continent diversions can lead to hydroureteronephrosis (pathologic dilation of the renal pelvis and ureter in response to slow draining of the collecting system) and renal dysfunction, and recurrent urinary tract infection.75

Bladder-Sparing Approaches

A regimen of maximal TURBT followed by radiosensitizing chemotherapy and radiation, dubbed “trimodal therapy” (TMT), is an alternative to cystectomy (AUA, strong/B; EAU, strong/2b) for patients who decline or are ill suited for surgery.4,7 Chemotherapy used in TMT is often combination cisplatin with fluorouracil or paclitaxel, or fluorouracil with mitomycin C, or cisplatin-alone (NCCN, preferred/2A) or low-dose gemcitabine (NCCN, other/2B), and functions both as a radiosensitizing agent as well as systemic treatment for any micrometastatic disease.8 In the absence of RCTs, large population- and hospital-based registry studies suggest poorer overall survival of TMT relative to cystectomy.76,77 However, a systematic review demonstrated that 5-year overall survival rates of 48% to 60% can be reached with TMT with prompt salvage cystectomy, if indicated.78 Partial cystectomy performed for curative intent may also be appropriate for a select group of patients.79 Several studies are now evaluating the potential synergy between radiation therapy and immunotherapy, including SWOG/RTG 1806, a phase 3 randomized study of conventional TMT with and without atezolizumab, a PD-L1 inhibitor (NCT03775265). Given the morbidity and effect on quality of life associated with both radical cystectomy and TMT, efforts are under way to tailor therapy to tumor molecular profiles, with the goal of increasing treatment efficacy and perhaps organ preservation, as in the RETAIN (NCT02710734) and ALLIANCE (NCT03609216) trials.

Advanced and Metastatic Disease

Only 4% of patients with newly diagnosed bladder cancer present with metastatic disease. Metastatic bladder cancer has a poor prognosis, with a median survival with standard chemotherapy of approximately 13 to 15 months.80,81 For decades, the mainstay of treatment has been cisplatin-based cytotoxic chemotherapy (Table 5). Recent advances, however, have provided additional treatments such as immunotherapy, targeted therapy, and antibody-drug conjugates, as second- and third-line options and as first-line options for patients with poor performance status or renal dysfunction who are ineligible to receive cisplatin (Table 6).8

Adjuvant Therapy

The role of adjuvant chemotherapy in patients with adverse pathologic features such as extravesical extension or node-positive disease after cystectomy remains controversial because prospective data do not support its use. One phase 3 trial randomized 284 patients who underwent radical cystectomy with pT3/pT4/node-positive disease and found no overall survival benefit in immediate postoperative vs delayed salvage chemotherapy (median overall survival, 6.7 vs 4.6 years; HR, 0.78 [95% CI, 0.56-1.08]; P = .13) at median follow-up of 7 years (interquartile range, 5.2-8.7).82 In the absence of high-quality evidence, guidelines recommend considering cisplatin-based adjuvant chemotherapy for patients who have high-risk pathologic features and did not receive neoadjuvant chemotherapy.64

Cytotoxic Chemotherapy

Cisplatin is essential to maximize efficacy of cytotoxic chemotherapy regimens. Guidelines recommend gemcitabine + cisplatin or dose-dense MVAC as first-line treatment for patients with metastatic disease (EAU, strong/1b; NCCN, preferred/2A). Gemcitabine + cisplatin is generally preferred, especially for frail patients, given the improved adverse-effect profile compared with MVAC. This was demonstrated in a phase 3 randomized trial of 405 patients showing that those in the MVAC group had significantly higher rates of grade 3 and 4 mucositis, neutropenia, neutropenic fever, and neutropenic sepsis, despite equivalent oncologic outcomes.81 The rationale for dose-dense MVAC comes from a phase 2/3 study of 263 patients with locally advanced or metastatic urothelial cancer that showed an improved objective response rate (72% vs 58%), complete response rate (25% vs 11%), median progression-free survival (9.5 vs 8.1 months; HR, 0.73 [95% CI, 0.56-0.95]), and overall survival (15.1 vs 14.9 months; HR, 0.76 [95% CI, 0.58-0.99]) compared with standard MVAC. In cisplatin-ineligible patients, carboplatin is an inferior alternative and should not be used in cisplatin-fit patients (EAU, strong/2a). Carboplatin-gemcitabine is an option in cisplatin-ineligible patients (NCCN, preferred/2A) but was shown to have a relatively worse objective response rate (0.36 [95% CI, 0.30-0.42]) and median overall survival (range, 7.2-10.0 months) in a meta-analysis.93

Immunotherapy

The high mutational burden of bladder cancer renders it susceptible to immunotherapy, particularly with checkpoint inhibitors, monoclonal antibodies against programmed cell death-1 (PD-1) and its ligand, PD-L1. Checkpoint pathways are endogenous mechanisms regulating autoimmunity and can be exploited by cancer cells to evade the immune response.94 Since 2016, 5 checkpoint inhibitors have been approved for the treatment of bladder cancer at various stages of disease. In the KEYNOTE-045 phase 3 trial, 542 patients with advanced disease who progressed with first-line therapy were randomized to pembrolizumab (anti-PD-1) vs second-line chemotherapy. Patients receiving pembrolizumab experienced improved overall survival (10.3 vs 7.4 months; HR, 0.73 [95% CI, 0.59-0.91]; P = .002) at median follow-up of 14.1 months (range, 9.9–22.1). In KEYNOTE-052, a single-group phase 2 study, pembrolizumab as first-line therapy in cisplatin-ineligible patients yielded an objective response rate of 24%, with 5% complete responses.83,84 Despite promising phase 2 data, atezolizumab (anti-PD-L1) did not improve overall survival (11.1 vs 10.6 months; HR, 0.87 [95% CI, 0.63-1.21]; P = .41) in a phase 3 randomized trial (IMvigor211) of patients who progressed with platin-based therapy, when compared with second-line chemotherapy at median follow-up of 17.3 months (range, 0-24.5).85 Both drugs have been approved as second-line agents or as first-line agents for cisplatin-ineligible patients whose tumors reach threshold PD-L1 expression on immunohistologic staining. Avelumab and durvalumab (anti-PD-L1), and nivolumab (anti-PD-1) are also approved as second-line agents demonstrating clinical benefit in the advanced or metastatic setting.86-88 With the approval of newer agents, efforts to identify rational combinations and delineate sequence of treatment are under way.

Targeted Therapies and Antibody-Drug Conjugates

Fibroblast growth factor receptor (FGFR) is a receptor tyrosine kinase involved in cell proliferation, survival, and migration and is a target in bladder and upper tract urothelial cancer, particularly in luminal-subtype tumors. A recent phase 2 study (BLC2001) in 99 patients with locally advanced and metastatic disease who did not respond to prior therapy found a 40% objective response rate with oral erdafitinib, a pan-FGFR inhibitor, resulting in FDA approval in the second-line setting.89 Notably, FGFR mutations are more frequent in the upper tract (≈30%) than the bladder (≈14%), based on small sample size sequencing studies and Cancer Genome Atlas data.95

Similarly, antibody-drug conjugates exploit highly expressed tumor proteins as targets for drug delivery.96 One such agent, enfortumab vedotin, uses an anti-nectin-4 antibody linked to the microtubule-disrupting molecule monomethyl auristatin E. A phase 2 single-group study (EV-201) demonstrated an objective response rate of 44% among patients who progressed following treatment with chemotherapy and immunotherapy.90 This led to FDA approval of enfortumab vedotin in this dual-refractory setting. More recently, the FDA has granted breakthrough designation status to combination therapy involving enfortumab vedotin with pembrolizumab, which has shown an overall response rate of 73%, with 16% complete responses in the first-line metastatic setting.91 Early results with sacituzumab govitecan, an antibody-drug conjugate that links a topoisomerase inhibitor with an antibody for trophoblast cell surface marker 2, has shown a 29% overall response rate in a single-group phase 2 study in patients who progressed with chemotherapy and immunotherapy.92

Limitations

This review has several limitations. First, given the scope of the topic, a systematic review could not be performed. Second, we also intentionally limited our discussion to the most common forms of the disease. For example, we did not discuss urothelial carcinoma of the upper urinary tracts, which accounts for approximately 5% of cases. While often treated similarly, upper tract urothelial carcinoma has unique biologic and technical considerations that warrant consideration. Further, bladder cancer is predominantly of the pure urothelial carcinoma histology, although variants (pure and mixed) are found in up to 25% of patients with advanced disease. These variants, which are not extensively discussed in this review, are driven by distinct molecular pathways and thus carry unique management implications. Third, the treatment landscape for bladder cancer is rapidly changing, and future therapies that may soon be standard options could not be discussed within the scope of this review.

Conclusions

Improved understanding of the molecular biology and genetics of bladder cancer has evolved the way localized and advanced disease is diagnosed and treated. While intravesical BCG has remained the mainstay of therapy for intermediate and high-risk non–muscle-invasive bladder cancer, the therapeutic options for muscle-invasive and advanced disease has expanded to include immunotherapy with checkpoint inhibition, targeted therapies, and antibody-drug conjugates.

Section Editors: Edward Livingston, MD, Deputy Editor, and Mary McGrae McDermott, MD, Deputy Editor.

Submissions: We encourage authors to submit papers for consideration as a Review. Please contact Edward Livingston, MD, at Edward.livingston@jamanetwork.org or Mary McGrae McDermott, MD, at mdm608@northwestern.edu.

Corresponding Author: Karim Chamie, MD, MSHS, Institute of Urologic Oncology, Department of Urology, David Geffen School of Medicine at UCLA, 300 Stein Plaza, Ste 370, Los Angeles, CA 90095 (kchamie@mednet.ucla.edu).

Accepted for Publication: August 26, 2020.

Author Contributions: Drs Lenis and Chamie had full access to all of the data in the study and take responsibility for the integrity of the data and the accuracy of the data analysis.

Concept and design, acquisition, analysis, or interpretation of data, drafting of the manuscript, critical revision of the manuscript for important intellectual content, and supervision: All authors.

Conflict of Interest Disclosures: Dr Chamie reported serving as a consultant for UroGen Pharma and receiving research funding from Salix Pharma. No other disclosures were reported.

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